You can only enter one error value for each X. Prism doesn't know if it is a SE or SD or something else. You cannot enter two error values to create asymmetric X error bars. The X error values never enter into any calculations. What happens if you enter X values into the row title column of a Grouped (or XY) table? A Grouped table has a column for entry of row titles. Prism sees these as titles or labels, even if you enter numbers in every row. The table below if a Grouped table with no X column. Note that there is no heading "X" and that the labels (numbers) entered in that column are left justified (X values would be right justified). Prism doesn't "know" these labels are numbers, so doesn't use the value as a X coordinate.FlowJo v10 makes it easy to convert bivariate dot plots to univariate histograms with a click of a button! Also note that the values are equally spread across the X axis.
To view your plot as a histogram, simply click the drop-down menu on the left side of the Graph Window and select “Histogram” from the menu. Use histograms to view frequency distribution of your flow data, one parameter at a time. Histograms display a frequency distribution of the data versus fluorescence intensity or some other parameter (e.g. This can be helpful to quickly analyze whether your samples have normal, uniform, bimodal, or other type of distribution. 7-AAD) was used during staining, you can quickly evaluate whether you have high or low numbers of live cells (live cells will be largely 7-AAD negative). In contrast to dot plots or other bivariate displays, there are different types of options and gating tools for histograms. See the sections below for more information about histogram options and gating tools.
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